| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
An improved protocol for stable and efficient culturing of chicken primordial germ cells using small-molecule inhibitors |
|
言語 |
en |
| 作成者 |
Ezaki, Ryo
Hirose, Fumiya
Furusawa, Shuichi
Horiuchi, Hiroyuki
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
This is a post-peer-review, pre-copyedit version of an article published in Cytotechnology. The final authenticated version is available online at: https://doi.org/10.1007/s10616-020-00385-9 |
| 権利情報 |
|
|
権利情報 |
This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Chicken |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Primordial germ cells |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Small-molecule inhibitor |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
chicken vasa homolog |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
NANOG |
| 内容記述 |
|
|
内容記述 |
At present, the most reliable method for creating genetically modified chickens is the modification of the DNA sequence of primordial germ cells (PGCs). However, during embryogenesis, only a small number of chicken PGCs can be obtained. Therefore, in vitro PGC culturing is necessary to obtain sufficient cells for further genetic engineering. Previously reported PGC culturing methods lack versatility. We report here a new protocol for stable and efficient culturing of chicken PGCs using small-molecule inhibitors. The growth rate of PGCs was investigated following the addition of three small-molecule inhibitors, including blebbistatin, into the culture medium. Chicken PGC survival and proliferation rates increased after the addition of small-molecule inhibitors, compared with the untreated control. Blebbistatin was shown to be the most effective inducer of PGC growth. Long-term culturing of PGCs with blebbistatin maintained the morphology of typical PGCs, and these cells expressed marker proteins such as chicken vasa homolog (CVH) and NANOG. Additionally, PGCs transfected with a fluorescent protein gene were shown to migrate into the gonads of the recipient embryo, and progeny derived from PGCs cultured by this method were efficiently obtained. These results demonstrate that small-molecule inhibitors represent a useful tool for stable and efficient chicken PGC culturing. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This work was supported by the Japan Society for the Promotion of Science KAKENHI Grant Numbers 19H03107, and 19K22286. |
| 出版者 |
|
|
出版者 |
Springer |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1007/s10616-020-00385-9 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1007/s10616-020-00385-9 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0920-9069 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1573-0778 |
| 開始ページ |
|
|
開始ページ |
397 |
| 書誌情報 |
Cytotechnology
Cytotechnology
巻 72,
p. 397-405,
発行日 2020-02-29
|
| 旧ID |
51074 |
Cytotechnology_72_397.pdf (4.2 MB)
