| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Effects of insulin, triiodothyronine and fat soluble vitamins on adipocyte differentiation and LPL gene expression in the stromal-vascular cells of red sea bream, Pagrus major |
|
言語 |
en |
| 作成者 |
Oku, Hiromi
Tokuda, Masaharu
Okumura, Takuji
Umino, Tetsuya
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2006 Elsevier Ltd. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
adipocyte |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
stromal-vascular cells |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
differentiation |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
lipoprotein lipase |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
LPL |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
teleost |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
red sea bream |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Pagrus major |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
660 |
| 内容記述 |
|
|
内容記述 |
Various kinds of hormones including insulin, triiodothyronine (T3) and fat-soluble vitamins have been proposed as mediators of adipocyte differentiation in mammals. To investigate the factors which are responsible for fish adipocyte differentiation, we developed a serum-free culture system of stromal-vascular cells of red sea bream adipose tissue and examined the effects of insulin (bovine), T3, and fat-soluble vitamins (all-trans retinoic acid, retinyl acetate and 1,25-dihydroxyvitamin D3) on the differentiation-linked expression of the lipoprotein lipase (LPL) gene. As assessed by the increase in LPL gene expression after 3 day cultivation, like in mammalian adipocytes, insulin enhanced the adipocyte differentiation in a concentration-dependent manner. During 2 week cultivation, bovine insulin promoted lipid accumulation in differentiating adipocytes concentration-dependently until the terminal differentiation. These results indicate that the differentiation of fish adipocytes is inducible by insulin alone. T3 alone had no effect but enhanced the differentiation-linked LPL gene expression in the presence of insulin. Fat-soluble vitamins, unlike in mammalian adipocytes, did not show any significant effects. The method developed in this study should be of interest for the characterization of factors involved in fish adipocyte differentiation. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.cbpb.2006.03.008 |
| 関連情報 |
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|
識別子タイプ |
PMID |
|
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関連識別子 |
16716627 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.cbpb.2006.03.008 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1096-4959 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11045017 |
| 開始ページ |
|
|
開始ページ |
326 |
| 書誌情報 |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
巻 144,
号 3,
p. 326-333,
発行日 2006-07
|
| 旧ID |
17058 |
CBP_144_326.pdf (327.2 KB)
