{"created":"2025-02-21T03:28:05.834454+00:00","id":2006660,"links":{},"metadata":{"_buckets":{"deposit":"dc790ac5-6c6b-4fd9-ab82-8a9310a188bc"},"_deposit":{"created_by":41,"id":"2006660","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2006660"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02006660","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Genotyping and mapping assay of single-nucleotide polymorphisms in CYP3A5 using DNA-binding zinc(II) complexes","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kinoshita, Eiji","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kinoshita","familyNameLang":"en"}],"givenNames":[{"givenName":"Eiji","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kinoshita-Kikuta, Emiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kinoshita-Kikuta","familyNameLang":"en"}],"givenNames":[{"givenName":"Emiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nakashima, Hiromi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Nakashima","familyNameLang":"en"}],"givenNames":[{"givenName":"Hiromi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Koike, Tohru","creatorNameLang":"en"}],"familyNames":[{"familyName":"Koike","familyNameLang":"en"}],"givenNames":[{"givenName":"Tohru","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"CYP3A5","subitem_subject_scheme":"Other"},{"subitem_subject":"SNP","subitem_subject_scheme":"Other"},{"subitem_subject":"Mutation","subitem_subject_scheme":"Other"},{"subitem_subject":"Zinc(II) complex","subitem_subject_scheme":"Other"},{"subitem_subject":"Phos-tag","subitem_subject_scheme":"Other"},{"subitem_subject":"Cyclen","subitem_subject_scheme":"Other"},{"subitem_subject":"PAGE","subitem_subject_scheme":"Other"},{"subitem_subject":"Electrophoresis","subitem_subject_scheme":"Other"},{"subitem_subject":"460","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Objective: It is clinically important to detect the single-nucleotide polymorphism (SNP) of CYP3A5*3 (6986A>G) associated with enzymatic activity for drug metabolism. The aim of this study was to establish an accurate strategy for SNP screening. Design and methods: Polyacrylamide gel electrophoresis (PAGE) using zinc(II) complexes were applied for SNP detection. Genomic analyses of 19 healthy subjects were conducted by using both Zn2+-Phos-tag-PAGE and Zn2+-cyclen-PAGE methodologies. Results: Zn2+-Phos-tag PAGE permitted identification of the following allele genotypes: the A/A homozygote (CMA5*1/*1) in 3 individuals, the G/G homozygote (CMA5*3/*3) in 14 individuals, and the A/G heterozygote (CMA5*1/*3) in 2 individuals. Zn2+-cyclen PAGE demonstrated not only reproducibility of the genotyping but also existence of a novel heterozygous SNP (6929G>A) in the subject with CMA5*1/*1. Conclusion: We demonstrated reliable SNP genotyping and mapping in CYP3A5 using the combination method of Zn2+-Phos-tag PAGE and Zn2+-cyclen PAGE.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Pergamon"},{"subitem_publisher":"Elsevier Science Ltd"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0009-9120","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00607603","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"302"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"306","bibliographicPageStart":"302","bibliographicVolumeNumber":"43","bibliographic_titles":[{"bibliographic_title":"Clinical Biochemistry"},{"bibliographic_title":"Clinical Biochemistry"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.clinbiochem.2009.09.007","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.clinbiochem.2009.09.007","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"ClinBiochem_43_302.pdf","filesize":[{"value":"1019.1 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2006660/files/ClinBiochem_43_302.pdf"},"version_id":"e1aa711b-382d-4101-b338-4779b96933a5"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"29228"},"item_title":"Genotyping and mapping assay of single-nucleotide polymorphisms in CYP3A5 using DNA-binding zinc(II) complexes","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2006660","relation_version_is_last":true,"title":["Genotyping and mapping assay of single-nucleotide polymorphisms in CYP3A5 using DNA-binding zinc(II) complexes"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T08:06:47.456830+00:00"}