{"created":"2025-02-21T03:25:07.067656+00:00","id":2006563,"links":{},"metadata":{"_buckets":{"deposit":"3297c26c-0470-41f8-83ee-f2149a892a47"},"_deposit":{"created_by":41,"id":"2006563","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2006563"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02006563","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Role of the JNK pathway in thrombin-induced ICAM-1 expression in endothelial cells","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Miho, Narimasa","creatorNameLang":"en"}],"familyNames":[{"familyName":"Miho","familyNameLang":"en"}],"givenNames":[{"givenName":"Narimasa","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ishida, Takafumi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ishida","familyNameLang":"en"}],"givenNames":[{"givenName":"Takafumi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kuwada, Noriko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kuwada","familyNameLang":"en"}],"givenNames":[{"givenName":"Noriko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ishida, Mari","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ishida","familyNameLang":"en"}],"givenNames":[{"givenName":"Mari","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shimote, Keiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Shimote","familyNameLang":"en"}],"givenNames":[{"givenName":"Keiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tabuchi, Kumiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Tabuchi","familyNameLang":"en"}],"givenNames":[{"givenName":"Kumiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Oshima, Tetsuya","creatorNameLang":"en"}],"familyNames":[{"familyName":"Oshima","familyNameLang":"en"}],"givenNames":[{"givenName":"Tetsuya","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yoshizumi, Masao","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yoshizumi","familyNameLang":"en"}],"givenNames":[{"givenName":"Masao","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Chayama, Kazuaki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Chayama","familyNameLang":"en"}],"givenNames":[{"givenName":"Kazuaki","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2005 European Society of Cardiology"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"G proteins","subitem_subject_scheme":"Other"},{"subitem_subject":"gene expression","subitem_subject_scheme":"Other"},{"subitem_subject":"MAP kinase","subitem_subject_scheme":"Other"},{"subitem_subject":"signal transduction","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Objective: Thrombin induces leukocyte adherence to endothelial cells via increased expression of intercellular adhesion molecule-1 (ICAM-1). Although ICAM-1 expression is regulated by NF-kappa B, recent studies have suggested that additional signaling mechanisms may also be involved. The goal of this study was to determine whether mitogen-activated protein (MAP) kinases, including extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), and p38 MAP kinase (p38), mediate thrombin-induced ICAM-1 expression in endothelial cells.Methods: Western blot analysis using anti-ICAM-1 antibody and luciferase assays were performed in cultured endothelial cells after addition of signal transduction inhibitors or transfection of various gene constructs. JNK kinase activity was determined by a kinase assay using c-Jun as a substrate or by Western blot analysis with anti-phospho-JNK antibody.Results: Treatment of endothelial cells with the JNK-specific inhibitors, SP600125 or JNK inhibitory peptide 1 (JNKI1), resulted in a significant decrease in thrombin-induced ICAM-1 expression as demonstrated by Western blot analysis (67 +/- 3 0x1.535a8bfffb01p-890nd 72 +/- 7%, respectively). In contrast, inhibitors of MEK and p38 had only minimal effect. The combination of SP600125 and the NF-kappa B inhibitor, BAY 11-7082, resulted in complete inhibition of thrombin-induced ICAM-1 expression. The G(alpha q) inhibitor, YM-254890, inhibited thrombin-induced JNK activation and ICAM-1 expression. Dominant-negative Ras and Rac1, but not Rho, inhibited thrombin-induced JNK activation and ICAM-1 promoter activity. Finally, thrombin-induced JNK activation and ICAM-1 promoter activity were inhibited by beta ARK1ct (a G beta gamma subunit scavenger) and Csk.Conclusions: These data suggest that, in concert with NF-kappa B, JNK regulates thrombin-induced ICAM-1 expression by a mechanism that is dependent on G(alpha q), G beta gamma, Ras, Rac1 and the Src kinase family.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"AA0059904X","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"0008-6363","subitem_source_identifier_type":"ISSN"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"289"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-11-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"298","bibliographicPageStart":"289","bibliographicVolumeNumber":"68","bibliographic_titles":[{"bibliographic_title":"Cardiovascular Research"},{"bibliographic_title":"Cardiovascular Research"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.cardiores.2005.05.029","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"15979056","subitem_relation_type_select":"PMID"}},{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.cardiores.2005.05.029","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"CardioRes_68_289.pdf","filesize":[{"value":"1.6 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2006563/files/CardioRes_68_289.pdf"},"version_id":"efd145c2-7a9a-4ad7-b1ca-ebd355caf2f9"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"24937"},"item_title":"Role of the JNK pathway in thrombin-induced ICAM-1 expression in endothelial cells","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2006563","relation_version_is_last":true,"title":["Role of the JNK pathway in thrombin-induced ICAM-1 expression in endothelial cells"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T07:53:40.093159+00:00"}