| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Isolation and Biosynthesis of an Azoxyalkene Compound Produced by a Multiple Gene Disruptant of Streptomyces rochei |
|
言語 |
en |
| 作成者 |
Kunitake, Hirofumi
Hiramatsu, Takahiro
Kinashi, Haruyasu
Arakawa, Kenji
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
© 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim |
| 権利情報 |
|
|
権利情報 |
This is the peer reviewed version of the following article: CHEMBIOCHEM. 2015 Oct 12;16(15):2237-2243, which has been published in final form at https://doi.org/10.1002/cbic.201500393. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
| 権利情報 |
|
|
権利情報 |
This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
biosynthesis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
genetic engineering |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
azoxyalkene |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
natural products |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Streptomyces |
| 内容記述 |
|
|
内容記述 |
Streptomyces rochei 7434AN4 predominantly produces lankacidin and lankamycin under normal culture conditions, suggesting that other biosynthetic gene clusters for secondary metabolites are silent. To exploit silent metabolites of strain 7434AN4, we constructed mutant KA57, a multiple disruptant of the transcriptional repressor gene srrB together with the biosynthesis genes for both antibiotics. Mutant KA57 accumulated a compound (KA57-A) with a strong UV absorption at 235 nm, which was not detected in the parent strain or other mutants. Various spectroscopic analyses revealed that KA57-A is an azoxyalkene compound of a molecular formula of C10H20N2O3 with the R configuration at C-2. Biosynthesis of KA57-A was also studied by feeding with labeled acetates, amino acids, and 1-hexylamine. The hexenyl moiety (C1’-C6’) was derived from fatty acid, while the 3-amino-butan-1,2-diol moiety (C1-C4) was derived from C-2 of acetate (C1) and serine (C2-C4). Incorporation of [1,1-2H2]1-hexylamine indicated that C1’-C2’ dehydrogenation occurs at the final step of biosynthesis. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
アクセプト後にアブストラクト等変更あり。 |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This work was supported by a Grants‐in‐Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT), and a Noda Institute for Scientific Research Grant. |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Supporting information for this articleis available on the WWW under http://dx.doi.org/10.1002/cbic.201500393. |
| 出版者 |
|
|
出版者 |
Wiley‐VCH Verlag |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1002/cbic.201500393 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
26300120 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1002/cbic.201500393 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1439-4227 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1439-7633 |
| 開始ページ |
|
|
開始ページ |
2237 |
| 書誌情報 |
CHEMBIOCHEM
CHEMBIOCHEM
巻 16,
号 15,
p. 2237-2243,
発行日 2015-10-12
|
| 旧ID |
48657 |
Chembiochem_16_2237.pdf (557.7 KB)
