{"created":"2025-02-21T03:22:52.366936+00:00","id":2006487,"links":{},"metadata":{"_buckets":{"deposit":"d69e982d-4f9a-40d5-a576-e1f68e1018b6"},"_deposit":{"created_by":41,"id":"2006487","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2006487"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02006487","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Stability enhancement of cytochrome c through heme deprotonation and mutations","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sonoyama, Takafumi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Sonoyama","familyNameLang":"en"}],"givenNames":[{"givenName":"Takafumi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hasegawa, Jun","creatorNameLang":"en"}],"familyNames":[{"familyName":"Hasegawa","familyNameLang":"en"}],"givenNames":[{"givenName":"Jun","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Uchiyama, Susumu","creatorNameLang":"en"}],"familyNames":[{"familyName":"Uchiyama","familyNameLang":"en"}],"givenNames":[{"givenName":"Susumu","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nakamura, Shota","creatorNameLang":"en"}],"familyNames":[{"familyName":"Nakamura","familyNameLang":"en"}],"givenNames":[{"givenName":"Shota","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kobayashi, Yuji","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kobayashi","familyNameLang":"en"}],"givenNames":[{"givenName":"Yuji","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sambongi, Yoshihiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Sambongi","familyNameLang":"en"}],"givenNames":[{"givenName":"Yoshihiro","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2008 Elsevier B.V."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Circular dichroism spectroscopy","subitem_subject_scheme":"Other"},{"subitem_subject":"Cytochrome c","subitem_subject_scheme":"Other"},{"subitem_subject":"Hydrogen bond","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein stability","subitem_subject_scheme":"Other"},{"subitem_subject":"Heme propionate","subitem_subject_scheme":"Other"},{"subitem_subject":"460","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"The chemical denaturation of Pseudomonas aeruginosa cytochrome c551 variants was examined at pH 5.0 and 3.6. All variants were stabilized at both pHs compared with the wild-type. Remarkably, the variants carrying the F34Y and/or E43Y mutations were more stabilized than those having the F7A/V13M or V78I ones at pH 5.0 compared with at pH 3.6 by ~3.0 – 4.6 kJ/mol. Structural analyses predicted that the side chains of introduced Tyr-34 and Tyr-43 become hydrogen donors for the hydrogen bond formation with heme 17-propionate at pH 5.0, but less efficiently at pH 3.6, because the propionate is deprotonated at the higher pH. Our results provide an insight into a stabilization strategy for heme proteins involving variation of the heme electronic state and introduction of appropriate mutations.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier Science BV"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0301-4622","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00566084","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"37"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"41","bibliographicPageStart":"37","bibliographicVolumeNumber":"139","bibliographic_titles":[{"bibliographic_title":"Biophysical Chemistry"},{"bibliographic_title":"Biophysical Chemistry"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bpc.2008.09.020","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.bpc.2008.09.020","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"BiophysChem_139_37.pdf","filesize":[{"value":"520.5 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2006487/files/BiophysChem_139_37.pdf"},"version_id":"fb4c298a-2b41-4036-a2e4-476c97aee2f9"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"25951"},"item_title":"Stability enhancement of cytochrome c through heme deprotonation and mutations","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2006487","relation_version_is_last":true,"title":["Stability enhancement of cytochrome c through heme deprotonation and mutations"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T07:41:04.513829+00:00"}