{"created":"2025-02-21T03:22:35.174349+00:00","id":2006477,"links":{},"metadata":{"_buckets":{"deposit":"296673dc-5a7b-4647-98fa-8c1336e7d93e"},"_deposit":{"created_by":41,"id":"2006477","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2006477"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02006477","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Characterization and Mutational Analysis of a Two-Polypeptide Bacteriocin Produced by Citrus Iyo-Derived Lactobacillus brevis 174A","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Noda, Masafumi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Noda","familyNameLang":"en"}],"givenNames":[{"givenName":"Masafumi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Miyauchi, Rumi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Miyauchi","familyNameLang":"en"}],"givenNames":[{"givenName":"Rumi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Danshiitsoodol, Narandalai","creatorNameLang":"en"}],"familyNames":[{"familyName":"Danshiitsoodol","familyNameLang":"en"}],"givenNames":[{"givenName":"Narandalai","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Higashikawa, Fumiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Higashikawa","familyNameLang":"en"}],"givenNames":[{"givenName":"Fumiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kumagai, Takanori","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kumagai","familyNameLang":"en"}],"givenNames":[{"givenName":"Takanori","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Matoba, Yasuyuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Matoba","familyNameLang":"en"}],"givenNames":[{"givenName":"Yasuyuki","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sugiyama, Masanori","creatorNameLang":"en"}],"familyNames":[{"familyName":"Sugiyama","familyNameLang":"en"}],"givenNames":[{"givenName":"Masanori","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2015 The Pharmaceutical Society of Japan "}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"bacteriocin","subitem_subject_scheme":"Other"},{"subitem_subject":"Lactobacillus brevis","subitem_subject_scheme":"Other"},{"subitem_subject":"actic acid bacteria","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"In the present study, we isolated a lactic acid bacterium (LAB) from a citrus iyo fruit and identified it as Lactobacillus brevis. This plant-derived LAB strain, designated 174A, produces bacteriocin consisting of two polypeptides designated brevicin 174A-β and 174A-γ. Although each polypeptide itself displays antibacterial activity, the ability is enhanced 100 fold by mixing both polypeptides at a 1 : 1 ratio. Significantly, brevicin 174A inhibits even the growth of several pathogenic bacteria that are more resistant to a lantibiotic bacteriocin, nisin A, which is commonly utilized as a preservative added to foodstuffs. Structural analysis of the 174A bacteriocin using a program that predicts secondary structure suggests that both component polypeptides have a positively charged N-terminal region, as well as two cysteine residues in both the N- and C-terminals. Judging from a mutational analysis of the antibacterial polypeptides, these unique amino acid sequences of 174A-β might be important for the expression of the synergistic activity that occurs in the presence of the two polypeptides combined. ","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"The Pharmaceutical Society of Japan"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0918-6158","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1347-5215","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA10885497","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"1902"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12 ","bibliographicPageEnd":"1909 ","bibliographicPageStart":"1902","bibliographicVolumeNumber":"38","bibliographic_titles":[{"bibliographic_title":"Biological and Pharmaceutical Bulletin"},{"bibliographic_title":"Biological and Pharmaceutical Bulletin"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1248/bpb.b15-00505","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1248/bpb.b15-00505","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"26632181","subitem_relation_type_select":"PMID"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"38_b15-00505.pdf","filesize":[{"value":"724.3 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2006477/files/38_b15-00505.pdf"},"version_id":"9c118137-c9c7-466c-b21c-3744c03707f4"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"46814"},"item_title":"Characterization and Mutational Analysis of a Two-Polypeptide Bacteriocin Produced by Citrus Iyo-Derived Lactobacillus brevis 174A","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2006477","relation_version_is_last":true,"title":["Characterization and Mutational Analysis of a Two-Polypeptide Bacteriocin Produced by Citrus Iyo-Derived Lactobacillus brevis 174A"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T07:40:05.413717+00:00"}