| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Differential gene regulation by VIV and VV ions in the branchial sac, intestine, and blood cells of a vanadium-rich ascidian, Ciona intestinalis |
|
言語 |
en |
| 作成者 |
Kume, Satoshi
Ueki, Tatsuya
Matsuoka, Hiroki
Hamada, Mayuko
Satoh, Nori
Michibata, Hitoshi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) Springer Science+Business Media, LLC. 2012 |
| 権利情報 |
|
|
権利情報 |
The final publication is available at Springer via http://dx.doi.org/10.1007/s10534-012-9569-z. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Ascidian |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Vanadium |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Microarray |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Gene regulation |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Glutathione |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
Ascidians are hyperaccumulators that have been studied in detail. Proteins and genes involved in the accumulation process have been identified, but regulation of gene expression related to vanadium accumulation remains unknown. To gain insights into the regulation of gene expression by vanadium in a genome-wide manner, we performed a comprehensive study on the effect of excess vanadium ions on a vanadium-rich ascidian, Ciona intestinalis, using a microarray. RT-PCR and enzyme activity assay were performed from the perspective of redox and accumulation of metal ions in each tissue. Glutathione metabolism-related proteins were significantly up-regulated by VIV treatment. Several genes involved in the transport of vanadium and protons, such as Nramp and V-ATPase, were significantly up-regulated by VIV treatment. We observed significant up-regulation of glutathione synthesis and degradation pathways in the intestine and branchial sac. In blood cells, expression of Ci-Vanabin4, glutathione reductase activity, glutathione levels, and vanadium concentration increased after VIV treatment. VIV treatment induced significant changes related to vanadium exclusion, seclusion, and redox pathways in the intestine and branchial sac. It also induced an enhancement of the vanadium reduction and accumulation cascade in blood cells. These differential responses in each tissue in the presence of excess vanadium ions suggest that vanadium accumulation and reduction may have regulatory functions. This is the first report on the gene regulation by the treatment of vanadium-rich ascidians with excess vanadium ions. It provided much information for the mechanism of regulation of gene expression related to vanadium accumulation. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This work was supported in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (#21570077 to H. M. and #20570070 to T. U.) and a grant from the Toray Science Foundation (03-4402 to T. U.). |
| 出版者 |
|
|
出版者 |
Springer Netherlands |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1007/s10534-012-9569-z |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://doi.org/10.1007/s10534-012-9569-z |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0966-0844 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10834000 |
| 開始ページ |
|
|
開始ページ |
1037 |
| 書誌情報 |
BioMetals
BioMetals
巻 25,
号 5,
p. 1037-1050,
発行日 2012-10
|
| 旧ID |
39963 |
BioMetals_25_1037.pdf (687.5 KB)
