| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Exclusive Expression of Transketolase in the Vanadocytes of the Vanadium-Rich Ascidian, Ascidia sydneiensis samea |
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言語 |
en |
| 作成者 |
Ueki, Tatsuya
Uyama, Taro
Yamamoto, Kazuhiro
Kanamori, Kan
Michibata, Hitoshi
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| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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権利情報 |
Copyright (c) 2000 Elsevier Science B.V. |
| 主題 |
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主題Scheme |
Other |
|
主題 |
Chordates |
| 主題 |
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主題Scheme |
Other |
|
主題 |
Metal Accumulation |
| 主題 |
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主題Scheme |
Other |
|
主題 |
Redox |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
Gene Expression |
| 主題 |
|
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主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
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内容記述 |
Ascidians, especially those belonging to the Ascidiidae, are known to accumulate extremely high levels of vanadium in vanadocytes, one type of blood (coelomic) cell. Vanadium, which exists in the +5 oxidation state in seawater, is accumulated in the vanadocytes and reduced to the +3 oxidation state. We have been trying to characterize all of the polypeptides specific to vanadocytes and to specify the proteins that participate in the accumulation and reduction of vanadium. To date, we have localized three enzymes in vanadocytes: 6-phosphogluconate dehydrogenase (6-PGDH: EC 1.1.1.44), glucose-6-phosphate dehydrogenase (G6PDH: EC 1.1.1.49), and glycogen phosphorylase (GP: EC 2.4.1.1), all of which are involved in the pentose phosphate pathway. In the current study, we cloned a cDNA for transketolase, an essential and rate-limiting enzyme in the non-oxidative part of the pentose phosphate pathway, from vanadocytes. The cDNA encoded a protein of 624 amino acids, which showed 61.8 0dentity to the human adult-type transketolase gene product. By immunocytochemistry and immunoblot analyses, the transketolase was revealed to be a protein that was expressed only in vanadocytes and not in any of the more than ten other types of blood cell. This finding, taken together with the localized expression of the other three enzymes, strongly supports the hypothesis that the pentose phosphate pathway functions exclusively in vanadocytes. |
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言語 |
en |
| 出版者 |
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出版者 |
Elsevier Science B.V. |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
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出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
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識別子タイプ |
PMID |
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関連識別子 |
11072071 |
| 関連情報 |
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識別子タイプ |
DOI |
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|
関連識別子 |
10.1016/S0167-4781(00)00222-0 |
| 関連情報 |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
http://dx.doi.org/10.1016/S0167-4781(00)00222-0 |
| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0167-4781 |
| 収録物識別子 |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10506089 |
| 開始ページ |
|
|
開始ページ |
83 |
| 書誌情報 |
Biochimica et biophysica acta
Biochimica et biophysica acta
巻 1494,
p. 83-90,
発行日 2000
|
| 旧ID |
23 |
BBA_1494_83-90_2000.pdf (982.6 KB)
