| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Sequence variation of Vanabin2-like vanadium-binding proteins in blood cells of the vanadium-accumulating ascidian Ascidia sydneiensis samea |
|
言語 |
en |
| 作成者 |
Ueki, Tatsuya
Satake, Makoto
Kamino, Kei
Michibata, Hitoshi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2008 Elsevier B.V. |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
vanadium |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
ascidian |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
metal binding protein |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
gene expression |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
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|
内容記述 |
The blood cells of ascidians accumulate extremely high levels of the transition metal vanadium. We previously isolated four vanadium-binding proteins (Vanabins 1–4) and a homologous protein (VanabinP) from the vanadium-rich ascidian Ascidia sydneiensis samea. In the present study, we identified cDNAs encoding five different Vanabin2-related proteins in A. sydneiensis samea blood cells. It was notable that the sequences of the encoded proteins vary from that of Vanabin2 at up to 14 specific positions, while both the polypeptide length and the 18 cysteine residues were completely conserved. The most divergent protein, named 14MT, differed from Vanabin2 at all 14 positions. Using immobilized metal-ion affinity chromatography, we found that Vanabin2 and 14MT have the same metal ion selectivity, but the overall affinity of 14MT for VO2+ is higher than that of Vanabin2. Binding number for VO2+ ions was same between Vanabin2 and 14MT as assessed by gel filtration. These results suggested that sequence variations were under strict evolutionary constraints and high affinity binding sites for VO2+ are conserved among Vanabin2 variants. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier Science BV |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.bbagen.2008.04.001 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.bbagen.2008.04.001 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0304-4165 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00564679 |
| 開始ページ |
|
|
開始ページ |
1010 |
| 書誌情報 |
Biochimica et Biophysica Acta (BBA) - General Subjects
Biochimica et Biophysica Acta (BBA) - General Subjects
巻 1780,
号 7-8,
p. 1010-1015,
発行日 2008-07
|
| 旧ID |
25974 |
BiochimBiophysActa-GenSubj_1780_1010.pdf (856.4 KB)
