{"created":"2025-02-21T03:19:36.482162+00:00","id":2006379,"links":{},"metadata":{"_buckets":{"deposit":"b518dd93-44d3-4116-8e02-562b0bed3718"},"_deposit":{"created_by":41,"id":"2006379","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2006379"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02006379","sets":["1730444907710"]},"author_link":[],"control_number":"2006379","item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Ceramide sorting into non-vesicular transport is independent of acyl chain length in budding yeast","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Schlarmann, Philipp","creatorNameLang":"en"}],"familyNames":[{"familyName":"Schlarmann","familyNameLang":"en"}],"givenNames":[{"givenName":"Philipp","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hanaoka, Kazuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Hanaoka","familyNameLang":"en"}],"givenNames":[{"givenName":"Kazuki","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ikeda, Atsuko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ikeda","familyNameLang":"en"}],"givenNames":[{"givenName":"Atsuko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Muñiz, Manuel","creatorNameLang":"en"}],"familyNames":[{"familyName":"Muñiz","familyNameLang":"en"}],"givenNames":[{"givenName":"Manuel","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Funato, Kouichi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Funato","familyNameLang":"en"}],"givenNames":[{"givenName":"Kouichi","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_rights_language":"en"},{"subitem_rights":"This is not the published version. Please cite only the published version.","subitem_rights_language":"en"},{"subitem_rights":"この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。","subitem_rights_language":"ja"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Ceramide sorting","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Endoplasmic reticulum","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Golgi","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Acyl chain length","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Budding yeast","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"The transport of ceramide from the endoplasmic reticulum (ER) to the Golgi is a key step in the synthesis of complex sphingolipids, the main building blocks of the plasma membrane. In yeast, ceramide is transported to the Golgi either through ATP-dependent COPII vesicles of the secretory pathway or by ATP-independent non-vesicular transport that involves tethering proteins at ER-Golgi membrane contact sites. Studies in both mammalian and yeast cells reported that vesicular transport mainly carries ceramide containing very long chain fatty acids, while the main mammalian non-vesicular ceramide transport protein CERT only transports ceramides containing short chain fatty acids. However, if non-vesicular ceramide transport in yeast similarly favors short chain ceramides remained unanswered. Here we employed a yeast GhLag1 strain in which the endogenous ceramide synthase is replaced by the cotton-derived GhLag1 gene, resulting in the production of short chain C18 rather than C26 ceramides. We show that block of vesicular transport through ATP-depletion or the use of temperature-sensitive sec mutants caused a reduction in inositolphosphorylceramide (IPC) synthesis to similar extent in WT and GhLag1 backgrounds. Since the remaining IPC synthesis is a readout for non-vesicular ceramide transport, our results indicate that non-vesicular ceramide transport is neither blocked nor facilitated when only short chain ceramides are present. Therefore, we propose that the sorting of ceramide into non-vesicular transport is independent of acyl chain length in budding yeast.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"This work was supported by JSPS KAKENHI Grant Number 19H02922, 23H02175 and 23K26868 to K.F, 22K14863 to A.I. and grant PID2020-119505 GB-I00/AEI/10.13039/501100011033 to MM, and grants co-financed by the Junta de Andalucía and the ERDF PY20_01240 and US-1380893 to M.M.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186901218":{"attribute_name":"Funding Reference","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"19H02922","subitem_award_uri":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H02922/"},"subitem_award_titles":[{"subitem_award_title":"糖脂質リモデリングによる蛋白質のソーティング機構","subitem_award_title_language":"ja"}],"subitem_funder_names":[{"subitem_funder_name":"日本学術振興会","subitem_funder_name_language":"ja"}]},{"subitem_award_numbers":{"subitem_award_number":"23H02175","subitem_award_uri":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-23K26868/"},"subitem_award_titles":[{"subitem_award_title":"Mechanisms of protein sorting by glycolipid remodeling  Research Project","subitem_award_title_language":"ja"}],"subitem_funder_names":[{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]},{"subitem_award_numbers":{"subitem_award_number":"22K14863","subitem_award_uri":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-22K14863/"},"subitem_award_titles":[{"subitem_award_title":"脂質ドメイン構成因子の非小胞輸送機構とマルチ輸送系の存在意義の解明","subitem_award_title_language":"ja"}],"subitem_funder_names":[{"subitem_funder_name":"日本学術振興会","subitem_funder_name_language":"ja"}]},{"subitem_award_titles":[{"subitem_award_title":"Mechanism of nonvesicular lipid domain component transport and significance of multi-transport systems","subitem_award_title_language":"ja"}],"subitem_funder_names":[{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]},{"subitem_award_titles":[{"subitem_award_title":"セラミド非小胞輸送に関与する新規タンパク質の同定と機能解明"}],"subitem_funder_names":[{"subitem_funder_name":"日本学術振興会","subitem_funder_name_language":"ja"}]},{"subitem_award_titles":[{"subitem_award_title":"セラミド非小胞輸送に関与する新規タンパク質の同定と機能解明"}],"subitem_funder_names":[{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"149980"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-04-23","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"149980","bibliographicVolumeNumber":"715","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"Resource 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