| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
In situ activity and spatial organization of anaerobic ammonium-oxidizing (anammox) bacteria in biofilms. |
|
言語 |
en |
| 作成者 |
Kindaichi, Tomonori
Tsushima, Ikuo
Ogasawara, Yuji
Shimokawa, Masaki
Ozaki, Noriatsu
Satoh, Hisashi
Okabe, Satoshi
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| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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|
権利情報 |
Copyright (c) American Society for Microbiology |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
510 |
| 内容記述 |
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内容記述 |
We investigated autotrophic anaerobic ammonium-oxidizing (anammox) biofilms for their spatial organization, community composition, and in situ activities by using molecular biological techniques combined with microelectrodes. Results of phylogenetic analysis and fluorescence in situ hybridization (FISH) revealed that ""Brocadia""-like anammox bacteria that hybridized with the Amx820 probe dominated, with 60 to 92% of total bacteria in the upper part (<1,000 ?m) of the biofilm, where high anammox activity was mainly detected with microelectrodes. The relative abundance of anammox bacteria decreased along the flow direction of the reactor. FISH results also indicated that Nitrosomonas-, Nitrosospira-, and Nitrosococcus-like aerobic ammonia-oxidizing bacteria (AOB) and Nitrospira-like nitrite-oxidizing bacteria (NOB) coexisted with anammox bacteria and accounted for 13 to 21% of total bacteria in the biofilms. Microelectrode measurements at three points along the anammox reactor revealed that the NH4+ and NO2? consumption rates decreased from 0.68 and 0.64 ?mol cm?2 h?1 at P2 (the second port, 170 mm from the inlet port) to 0.30 and 0.35 ?mol cm?2 h?1 at P3 (the third port, 205 mm from the inlet port), respectively. No anammox activity was detected at P4 (the fourth port, 240 mm from the inlet port), even though sufficient amounts of NH4+ and NO2? and a high abundance of anammox bacteria were still present. This result could be explained by the inhibitory effect of organic compounds derived from biomass decay and/or produced by anammox and coexisting bacteria in the upper parts of the biofilm and in the upstream part of the reactor. The anammox activities in the biofilm determined by microelectrodes reflected the overall reactor performance. The several groups of aerobic AOB lineages, Nitrospira-like NOB, and Betaproteobacteria coexisting in the anammox biofilm might consume a trace amount of O2 or organic compounds, which consequently established suitable microenvironments for anammox bacteria. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
American Society for Microbiology |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1128/AEM.00156-07 |
| 関連情報 |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1128/AEM.00156-07 |
| 収録物識別子 |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0099-2240 |
| 収録物識別子 |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00543249 |
| 開始ページ |
|
|
開始ページ |
4931 |
| 書誌情報 |
Applied and Environmental Microbiology
Applied and Environmental Microbiology
巻 73,
号 15,
p. 4931-4939,
発行日 2007-08
|
| 旧ID |
20778 |
AEM_73_4931.pdf (825.7 KB)
