| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
A continuous-flow ATP amplification system for increasing the sensitivity of quantitative bioluminescence assay |
|
言語 |
en |
| 作成者 |
Satoh, Tetsuya
Shinoda, Yasuharu
Alexandrov, Maxym
Kuroda, Akio
Murakami, Yuji
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2008 Elsevier Inc. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Bioluminescence assay |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
ATP amplification |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Continuous-flow system |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
We constructed a novel ATP amplification reactor using a continuous-flow system, and this allowed us to increase the sensitivity of quantitative bioluminescence assay by controlling the number of ATP amplification cycles. We previously developed a bioluminescence assay coupled with ATP amplification using a batch system. However, it was difficult to control the number of amplification cycles. In this study, ATP amplification was performed using a continuous-flow system, and significant linear correlations between amplified luminescence and initial ATP concentration were observed. When performing 4 cycles of continuous-flow ATP amplification, the gradient of amplification was 1.87N. Whereas lower quantifiable level was 500 pM without amplification, values as low as 50 pM ATP could be measured after amplification. Sensitivity thus increased 10-fold, with further improvements expected with additional amplification cycles. The continuous-flow system thus effectively increased the sensitivity of quantitative bioluminescence assay. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Academic Press Inc. |
| 出版者 |
|
|
出版者 |
Elsevier Science |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.ab.2008.04.045 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.ab.2008.04.045 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0003-2697 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00524867 |
| 開始ページ |
|
|
開始ページ |
116 |
| 書誌情報 |
Analytical Biochemistry
Analytical Biochemistry
巻 379,
号 1,
p. 116-120,
発行日 2008-08-01
|
| 旧ID |
25967 |
AnalBiochem_379_116.pdf (1.2 MB)
