{"created":"2025-02-21T03:14:02.332980+00:00","id":2006206,"links":{},"metadata":{"_buckets":{"deposit":"7072a35f-9719-4c09-9489-6cddfa8a11ef"},"_deposit":{"created_by":41,"id":"2006206","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2006206"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02006206","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"A mobility-shift detection method for DNA methylation analysis using phosphate-affinity polyacrylamide gel electrophoresis","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kinoshita-Kikuta, Emiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kinoshita-Kikuta","familyNameLang":"en"}],"givenNames":[{"givenName":"Emiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kinoshita, Eiji","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kinoshita","familyNameLang":"en"}],"givenNames":[{"givenName":"Eiji","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Koike, Tohru","creatorNameLang":"en"}],"familyNames":[{"familyName":"Koike","familyNameLang":"en"}],"givenNames":[{"givenName":"Tohru","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2008 Elsevier Inc."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"DNA methylation; Epigenetics","subitem_subject_scheme":"Other"},{"subitem_subject":"Methylation-specific PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"Phosphate-affinity PAGE","subitem_subject_scheme":"Other"},{"subitem_subject":"Phos-tag","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Herein, we describe a procedure for DNA methylation analysis using the bisulfite-mediated C-to-U conversion of a target DNA followed by methylation-specific polymerase chain reaction (MSP) and phosphate-affinity polyacrylamide gel electrophoresis (PAGE). The MSP was performed using a 1:1 mixture of 5'-phosphorylated methylation-specific and 5'-OH non-methylation-specific primers. The PAGE using an immobilized phosphate-binding tag molecule (i.e., a polyacrylamide-bound dizinc(II) complex, Zn2+–Phos-tag), which selectively captures the 5'-phosphorylated DNA fragment, enabled the mobility-shift detection of the methylation-specific product as a slower migration band. Using this novel procedure, we demonstrated the detection of a methylated cytosine base in a pUC19 plasmid.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Academic Press Inc."},{"subitem_publisher":"Elsevier Science"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0003-2697","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00524867","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"102"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-07-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"104","bibliographicPageStart":"102","bibliographicVolumeNumber":"378","bibliographic_titles":[{"bibliographic_title":"Analytical Biochemistry"},{"bibliographic_title":"Analytical Biochemistry"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ab.2008.03.021","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.ab.2008.03.021","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"AnalBiochem_378_102.pdf","filesize":[{"value":"635.0 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2006206/files/AnalBiochem_378_102.pdf"},"version_id":"a152d1f7-9b8d-48ab-9f81-b10c0c93ad7b"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"25928"},"item_title":"A mobility-shift detection method for DNA methylation analysis using phosphate-affinity polyacrylamide gel electrophoresis","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2006206","relation_version_is_last":true,"title":["A mobility-shift detection method for DNA methylation analysis using phosphate-affinity polyacrylamide gel electrophoresis"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T06:56:50.397512+00:00"}