{"created":"2025-02-21T03:14:00.422656+00:00","id":2006205,"links":{},"metadata":{"_buckets":{"deposit":"e6714d0e-0b91-4696-8fac-bca72c2ec324"},"_deposit":{"created_by":41,"id":"2006205","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2006205"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02006205","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"A single nucleotide polymorphism genotyping method using phosphate-affinity polyacrylamide gel electrophoresis","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kinoshita, Eiji","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kinoshita","familyNameLang":"en"}],"givenNames":[{"givenName":"Eiji","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kinoshita-Kikuta, Emiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kinoshita-Kikuta","familyNameLang":"en"}],"givenNames":[{"givenName":"Emiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Koike, Tohru","creatorNameLang":"en"}],"familyNames":[{"familyName":"Koike","familyNameLang":"en"}],"givenNames":[{"givenName":"Tohru","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2007 Elsevier Ltd."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"SNP genotyping","subitem_subject_scheme":"Other"},{"subitem_subject":"Allele-specific PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"Phosphate-affinity PAGE","subitem_subject_scheme":"Other"},{"subitem_subject":"Zinc","subitem_subject_scheme":"Other"},{"subitem_subject":"460","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"To date, various methods have been developed to facilitate the genotyping of a single nucleotide polymorphism (SNP) for aiding in the diagnosis and treatment of inherited diseases. The most commonly used method for SNP genotyping is an allele-specific hybridization procedure using an expensive fluorochrome-labeled oligonucleotide probe and a specialized fluorescence analyzer. Here, we introduce a simple and reliable genotyping method using a 1:1 mixture of 5'-phosphate-labeled and non-labeled allele-specific PCR primers. The method is based on the difference in mobility of the phosphorylated and nonphosphorylated PCR products (in the same number of base pairs) on phosphate-affinity polyacrylamide gel electrophoresis. The phosphate-affinity site is a polyacrylamide-bound dinuclear zinc(II) complex, which preferentially captures the 5'-phosphate-labeled allele-specific product compared with the corresponding non-labeled product. The obtained DNA migration bands can be visualized by ethidium bromide staining. We demonstrate the genotyping of a SNP reported in a human cardiac sodium channel gene, SCN5A, using this novel procedure.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0003-2697","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00524867","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"294"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-02-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"298","bibliographicPageStart":"294","bibliographicVolumeNumber":"361","bibliographic_titles":[{"bibliographic_title":"Analytical Biochemistry"},{"bibliographic_title":"Analytical Biochemistry"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ab.2006.11.032","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"17196923","subitem_relation_type_select":"PMID"}},{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.ab.2006.11.032","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"AnalBiochem_361_294.pdf","filesize":[{"value":"312.8 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2006205/files/AnalBiochem_361_294.pdf"},"version_id":"cbc06b0f-c35d-458f-9266-c00387fa142f"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"17037"},"item_title":"A single nucleotide polymorphism genotyping method using phosphate-affinity polyacrylamide gel electrophoresis","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2006205","relation_version_is_last":true,"title":["A single nucleotide polymorphism genotyping method using phosphate-affinity polyacrylamide gel electrophoresis"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T06:56:44.604206+00:00"}