{"created":"2025-02-21T03:13:56.488555+00:00","id":2006203,"links":{},"metadata":{"_buckets":{"deposit":"07e6b7ac-ccdc-491a-a154-5405ad91e9b6"},"_deposit":{"created_by":41,"id":"2006203","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2006203"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02006203","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"A heteroduplex-preferential Tm depressor for the specificity-enhanced DNA polymerase chain reactions","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kinoshita, Eiji","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kinoshita","familyNameLang":"en"}],"givenNames":[{"givenName":"Eiji","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kinoshita-Kikuta, Emiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kinoshita-Kikuta","familyNameLang":"en"}],"givenNames":[{"givenName":"Emiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Koike, Tohru","creatorNameLang":"en"}],"familyNames":[{"familyName":"Koike","familyNameLang":"en"}],"givenNames":[{"givenName":"Tohru","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2004 Elsevier Ltd."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Macrocyclic tetraamine","subitem_subject_scheme":"Other"},{"subitem_subject":"Zinc(II) complex","subitem_subject_scheme":"Other"},{"subitem_subject":"Tm depressor","subitem_subject_scheme":"Other"},{"subitem_subject":"Specificity-enhanced PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"460","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"A macrocyclic tetraamine zinc(II) complex appended with two quinoline groups, Zn2+.1,7-bis(4-quinolylmethyl)-1,4,7,10-tetraazacyclododecane (Zn2+.Q2-cyclen), was successfully used as a novel additive to suppress non-specific products in DNA polymerase chain reaction (PCR). In the presence of Zn2+.Q2-cyclen, the Tm drop of 20-bp heteroduplexes containing a non-complementary base pair was greater than that of the corresponding homoduplex (i.e., primer DNA). Here, we applied such preferential DNA melting to a specificity-enhanced PCR using micromolar concentrations of Zn2+.Q2-cyclen. We demonstrated the selective amplification of target DNA fragments (i.e., the human heart sodium channel Nav1.5 gene) from genomic DNA or a cDNA library. The optimum condition for the specificity-enhanced PCR could be determined in the concentration range of 1.50 μM of Zn2+.Q2-cyclen.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0003-2697","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00524867","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"154"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-02-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"160","bibliographicPageStart":"154","bibliographicVolumeNumber":"337","bibliographic_titles":[{"bibliographic_title":"Analytical Biochemistry"},{"bibliographic_title":"Analytical Biochemistry"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ab.2004.10.009","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"15649389","subitem_relation_type_select":"PMID"}},{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.ab.2004.10.009","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"AnalBiochem_337_154.pdf","filesize":[{"value":"565.3 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2006203/files/AnalBiochem_337_154.pdf"},"version_id":"5d8cc509-5c35-47be-b70f-03af8ad50d78"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"17036"},"item_title":"A heteroduplex-preferential Tm depressor for the specificity-enhanced DNA polymerase chain reactions","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2006203","relation_version_is_last":true,"title":["A heteroduplex-preferential Tm depressor for the specificity-enhanced DNA polymerase chain reactions"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T06:56:33.387820+00:00"}