| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Evaluation of recombinant MGL_1304 produced by Pichia pastoris for clinical application to sweat allergy |
|
言語 |
en |
| 作成者 |
Kan, Takanobu
Hiragun, Takaaki
Ishii, Kaori
Hiragun, Makiko
Yanase, Yuhki
Tanaka, Akio
Hide, Michihiro
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright © 2015, Japanese Society of Allergology. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/ licenses/by-nc-nd/4.0/) |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
HRT |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
MGL_1304 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Pichia pastoris |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Sweat allergy |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
β-hexosaminidase release |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
Background: We previously identified MGL_1304 secreted by Malassezia globosa as a sweat antigen for patients with atopic dermatitis (AD) and cholinergic urticaria (ChU). However, purifying native MGL_1304 from human sweat or culture supernatant of M. globosa (sup-MGL_1304) is costly and timeconsuming. Moreover, recombinant MGL_1304 expressed by using Escherichia coli (TF-rMGL_1304) needs a large chaperon protein and lacks the original glycosylation of yeasts. Thus, we generated a recombinant MGL_1304 by Pichia pastoris (P-rMGL_1304) and investigated its characteristic features. Methods: Recombinant MGL_1304 proteins expressed by E. coli and P. pastoris were generated. Properties of these recombinants and native antigens were compared by western blot analysis, histamine release tests (HRT) of patients with AD and ChU, and b-hexosaminidase release tests with RBL-48 cells. PrMGL_ 1304-specific IgE in sera of patients with AD were measured by sandwich ELISA. Results: Western blot analysis revealed that IgE of patients with AD bound to all MGL_1304 recombinants and native antigens. The histamine releasing ability of P-rMGL_1304 was 100 times higher than that of TF-rMGL_1304, and was comparable to that of sup-MGL_1304. Degranulation rates of RBL-48 cells, sensitized with sera of patients with AD in response to the stimulation of P-rMGL_1304, were comparable to those of sup-MGL_1304, whereas those of TF-rMGL_1304 were relatively weak. The levels of P-rMGL_1304-specific IgE in sera of patients with AD were correlated with their disease severities. Conclusions: P-rMGL_1304 has an antigenicity comparable to the native antigen, and is more useful than TF-rMGL_1304, especially in HRT and degranulation assay of RBL-48 cells. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This work was supported in part by the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT/ JSPS KAKENHI, Grant numbers 19790789, 22591239, 25461669), Grant-in-Aid for Young Scientists (B), and Scientific Research (C); and Health and Labour Sciences Research Grant; A-STEP program of Japan Science and Technology Agency. |
| 出版者 |
|
|
出版者 |
Japanese Society of Allergology |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.alit.2015.03.003 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
26117259 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.alit.2015.03.003 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1323-8930 |
| 開始ページ |
|
|
開始ページ |
266 |
| 書誌情報 |
Allergology International
Allergology International
巻 64,
号 3,
p. 266-271,
発行日 2015-07
|
| 旧ID |
47022 |
AI_64_266.pdf (791.9 KB)
