| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Development of butanol-tolerant Bacillus subtilis strain GRSW2-B1 as a potential bioproduction host |
|
言語 |
en |
| 作成者 |
Kataoka, Naoya
Tajima, Takahisa
Katou, Junichi
Rachadech, Wanitcha
Alisa, S Vangnai
|
| アクセス権 |
|
|
アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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|
権利情報 |
© 2011 Kataoka et al; licensee Springer. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
Organic-solvent tolerant bacteria |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
Butanol-tolerant bacteria |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Heterologous gene-expression host |
| 内容記述 |
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|
内容記述 |
As alternative microbial hosts for butanol production with organic-solvent tolerant trait are in high demands, a butanol-tolerant bacterium, Bacillus subtilis GRSW2-B1, was thus isolated. Its tolerance covered a range of organic solvents at high concentration (5%v/v), with remarkable tolerance in particular to butanol and alcohol groups. It was susceptible for butanol acclimatization, which resulted in significant tolerance improvement. It has versatility for application in a variety of fermentation process because it has superior tolerance when cells were exposed to butanol either as high-density, late-exponential grown cells (up to 5%v/v) or under growing conditions (up to 2.25%v/v). Genetic transformation procedure was optimized, yielding the highest efficiency at 5.17 × 103 colony forming unit (μg DNA)-1. Gene expression could be effectively driven by several promoters with different levels, where as the highest expression was observed with a xylose promoter. The constructed vector was stably maintained in the transformants, in the presence or absence of butanol stress. Adverse effect of efflux-mediated tetracycline resistance determinant (TetL) to bacterial organic-solvent tolerance property was unexpectedly observed and thus discussed. Overall results indicate that B. subtilis GRSW2-B1 has potential to be engineered and further established as a genetic host for bioproduction of butanol. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This work was the collaboration of Chulalongkorn University - Hiroshima University under the Asian Core Program (ACP) and financially supported by The Japan Society for the Promotion of Science (JSPS) and the National Research Council of Thailand (NRCT) (Bilateral Project). It was partly supported by the Thai Government Stimulus Package 2 (TKK2555) under the Project for Establishment of Comprehensive Center for Innovative Food, Health Products and Agriculture (PERFECTA). |
| 出版者 |
|
|
出版者 |
Springer Nature |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1186/2191-0855-1-10 |
| 関連情報 |
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
21906347 |
| 関連情報 |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1186/2191-0855-1-10 |
| 収録物識別子 |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2191-0855 |
| 開始ページ |
|
|
開始ページ |
10 |
| 書誌情報 |
AMB Express
AMB Express
巻 1,
号 1,
p. 10,
発行日 2011-05-30
|
| 旧ID |
46813 |
2191-0855-1-10.pdf (314.5 KB)
