{"created":"2025-02-21T01:33:43.550385+00:00","id":2005894,"links":{},"metadata":{"_buckets":{"deposit":"acb0ffe6-430b-462b-b1bb-f018a7214a33"},"_deposit":{"created_by":41,"id":"2005894","owner":"41","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2005894"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02005894","sets":["1730444908512:1730444916333"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Δ[4]-3-ケトステロイド5β-還元酵素に関する研究","subitem_title_language":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大西, 芳秋","creatorNameLang":"ja"},{"creatorName":"Onishi, Yoshiaki","creatorNameLang":"en"}],"familyNames":[{"familyName":"大西","familyNameLang":"ja"},{"familyName":"Onishi","familyNameLang":"en"}],"givenNames":[{"givenName":"芳秋","givenNameLang":"ja"},{"givenName":"Yoshiaki","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright(c) by Author"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Δ^4-3-ケトステロイド5β-還元酵素の免疫化学的解析,タンパク化学的解析および分子生物学的解析を行ったことにより以下の知見を得た。  1. 本酵素に特異的なモノクローナル抗体Mab-5βを作成した。  2. 本酵素のN末端アミノ酸は封鎖されていることが示唆された。  3. 本酵素をタンパク化学的に純粋なものとし,ペプチド化することによりタンパクの内部構造を決定した。  4. 本酵素のcDNAクローニングを行いその全構造を決定した。  5. COS細胞におけるタンパク発現実験を行いΔ^4-3-ケトステロイド5β-還元酵素とコルチゾン5β-還元酵素は同じ酵素であることを分子生物学的に証明した。  6. 本酵素が肝に局在していることを示した。  7. 本酵素の活性発現はPosttranslationalに調節されていることが示唆された。","subitem_description_language":"ja"},{"subitem_description":"目次  略語 / p1  序言 / p2  解析方針 / p4  1・免疫化学的解析 / p5  　概要 / p5  　材料ならびに方法 / p5  　結果 / p6  2・タンパク化学的解析 / p13  　概要 / p13  　材料ならびに方法 / p13  　結果 / p14  3・分子生物学的解析 / p23  　概要 / p23  　材料ならびに方法 / p23  　結果 / p26  4・調節機構についての研究 / p38  　概要 / p38  　材料ならびに方法 / p38  　結果 / p38  考察 / p43  総括 / p46  試薬 / p47  参考文献 / p48","subitem_description_type":"TableOfContents"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_1617186819068":{"attribute_name":"Identifier Registration","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.11501/3055118","subitem_identifier_reg_type":"JaLC"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"1"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicPageStart":"1"}]},"item_1617187087799":{"attribute_name":"Dissertation Number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第967号"}]},"item_1617187112279":{"attribute_name":"Degree Name","attribute_value_mlt":[{"subitem_degreename":"博士(歯学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Dentistry","subitem_degreename_language":"en"}]},"item_1617187136212":{"attribute_name":"Date Granted","attribute_value_mlt":[{"subitem_dategranted":"1991-03-25"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"diss_ko967.pdf","filesize":[{"value":"13.1 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2005894/files/diss_ko967.pdf"},"version_id":"da774171-964e-473d-938b-94fd81387092"}]},"item_1617944105607":{"attribute_name":"Degree Grantor","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"広島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"15401","subitem_degreegrantor_identifier_scheme":"kakenhi"}]},{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Hiroshima University"}]}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"31773"},"item_title":"Δ[4]-3-ケトステロイド5β-還元酵素に関する研究","item_type_id":"40003","owner":"41","path":["1730444916333"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2005894","relation_version_is_last":true,"title":["Δ[4]-3-ケトステロイド5β-還元酵素に関する研究"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T01:34:01.776405+00:00"}