WEKO3
アイテム
活性酸素種曝露による血管内皮細胞単層膜のアルブミン透過性 : 細胞膜カルシウムチャネルの可逆的な機能変化
https://hiroshima.repo.nii.ac.jp/records/2005333
https://hiroshima.repo.nii.ac.jp/records/2005333fe905949-9f5c-4b1b-9d89-e8ea4dfcffe6
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
diss_ko2110.pdf (689.9 KB)
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| アイテムタイプ | デフォルトアイテムタイプ_（フル）(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-03-18 | |||||||||
| タイトル | ||||||||||
| タイトル | Albumin permeability across endothelial cell monolayer exposed to reactive oxygen intermediates : Involvement of reversible functional alteration of the cell membrane Ca2+channels | |||||||||
| 言語 | en | |||||||||
| タイトル | ||||||||||
| タイトル | 活性酸素種曝露による血管内皮細胞単層膜のアルブミン透過性 : 細胞膜カルシウムチャネルの可逆的な機能変化 | |||||||||
| 言語 | ja | |||||||||
| 作成者 |
佐伯, 昇
× 佐伯, 昇
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| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 権利情報 | ||||||||||
| 権利情報 | Copyright (c) by Author | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Albumin permeability | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Reactive oxygen intermediates | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Calcium channels | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Redox state | |||||||||
| 主題 | ||||||||||
| 主題Scheme | NDC | |||||||||
| 主題 | 490 | |||||||||
| 内容記述 | ||||||||||
| 内容記述 | This study was designed to test the idea that the redox state of sulfhydryl (SH)-groups in cell-membrane Ca2+ channels plays a pivotal role in Ca2+in flux, which in turn causes an increase in albumin permeability across the cultured monolayer of porcine pulmonary artery endothelial (PPAE) cells exposed to xanthine/xanthine oxidase (X/XO). Albumin permeability as well as the concentration of intracellular Ca2+( [Ca2+]i) was increased by XKO. A Hz02 scavenger (catalase), an iron chelator (o-phenanthroline), and a hydroxyl radical scavenger (dimethyl sulfoxide) inhibited these changes provoked by XKO, in which intracellular iron-catalyzed hydroxyl radical generation was suggested to be involved. The increase in albumin permeability and [Ca2+]i continued once the PPAE cells were exposed to XKO. The [Ca2+]wi as decreased by a Ca2+ channel blocker, Ni2+, while the removal of Ni2+in creased [Ca2+]I again, suggesting the sustained Ca2+ influx through cell-membrane Ca" channels was responsible for the [Ca2+]i elevation. Ni2+f ailed to inhibit albumin permeability sustained after the removal of XKO. In contrast, SH-reducing agents (dithiothreitol and glutathione) inhibited the sustained permeability as well as Ca2+ influx. We concluded that the redox alteration of SH-groups in cell-membrane Ca2+ channels was involved in the increase in albumin permeability after exposure of the endothelial cells to oxidative stress. | |||||||||
| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | Hiroshima J. Med. Sci. Vol.49, No.1, 57-65, March, 2000 HIJM49-8 | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第2110号 | |||||||||
| 学位名 | ||||||||||
| 学位名 | 博士(医学) | |||||||||
| 言語 | ja | |||||||||
| 学位名 | ||||||||||
| 学位名 | Medicine | |||||||||
| 言語 | en | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2000-03-07 | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関識別子 | 15401 | |||||||||
| 学位授与機関名 | 広島大学 | |||||||||
| 言語 | ja | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関名 | Hiroshima University | |||||||||
| 言語 | en | |||||||||
| 旧ID | 17486 | |||||||||
