{"created":"2025-02-18T09:57:24.731225+00:00","id":2004963,"links":{},"metadata":{"_buckets":{"deposit":"1fab0fd3-dea8-43d7-b1d8-d04d0736cf36"},"_deposit":{"created_by":41,"id":"2004963","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2004963"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02004963","sets":["1730444908512:1730444915582"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Involvement of ribonucleotide reductase-M1 in 5-fluorouracil-induced DNA damage in esophageal cancer cell lines","subitem_title_language":"en"},{"subitem_title":"食道癌細胞株における5-フルオロウラシルにより誘導されるDNA障害へのリボヌクレオチドリダクターゼM1の関与","subitem_title_language":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"青木, 義朗","creatorNameLang":"ja"},{"creatorName":"Aoki, Yoshiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"青木","familyNameLang":"ja"},{"familyName":"Aoki","familyNameLang":"en"}],"givenNames":[{"givenName":"義朗","givenNameLang":"ja"},{"givenName":"Yoshiro","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright(c) by Author"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"ribonucleotide reductase","subitem_subject_scheme":"Other"},{"subitem_subject":"cisplatin","subitem_subject_scheme":"Other"},{"subitem_subject":"5-fluorouracil","subitem_subject_scheme":"Other"},{"subitem_subject":"γ-H2AX","subitem_subject_scheme":"Other"},{"subitem_subject":"RAD51","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"5-Fluorouracil (5-FU) is one of the most well established chemotherapeutic agents in the treatment of esophageal cancer. Ribonucleotide reductase M1 (RRM-1) is the rate?limiting enzyme in de novo DNA synthesis, and has been considered to play an important role in the 5-FU metabolic pathway. However, the means by which RRM-1 participates in the anticancer effects of 5-FU and cisplatin (CDDP) have not been well studied. Here, we show that RRM-1 significantly contributes to the induction of DNA damage by 5-FU in esophageal cancer cell lines. An assay of γ-H2AX focus forma-tion, a marker of DNA damage, after 5-FU treatment revealed good correlation with the levels of RRM-1 protein expression. Moreover, the increased sensitivity and RAD51 focus forma-tion induced by the combination treatment of 5-FU and CDDP were significantly repressed by RRM-1 depletion. These results suggest that RRM-1 is involved not only in the induction of DNA damage by 5-FU but also in the synergistic cytotoxic effect in the combination therapy of 5-FU and CDDP.","subitem_description_language":"en"}]},"item_1617186660861":{"attribute_name":"Date","attribute_value_mlt":[{"subitem_date_issued_datetime":"2014-11-21","subitem_date_issued_type":"Created"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617187087799":{"attribute_name":"Dissertation Number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第6190号"}]},"item_1617187112279":{"attribute_name":"Degree Name","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_1617187136212":{"attribute_name":"Date Granted","attribute_value_mlt":[{"subitem_dategranted":"2013-05-23"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"YOSHIRO AOKI, KENJI SAKOGAWA, JUN HIHARA, MANABU EMI, YOICHI HAMAI, KAZUTERU KONO, LIN SHI, JIYING SUN, HIROYUKI KITAO, TSUYOSHI IKURA, HIROYUKI NIIDA, MAKOTO NAKANISHI, MORIHITO OKADA and SATOSHI TASHIRO; Involvement of ribonucleotide reductase-M1 in 5-fluorouracil-induced DNA damage in esophageal cancer cell lines; INTERNATIONAL JOURNAL OF ONCOLOGY 42: 1951-1960, 2013 (DOI: 10.3892/ijo.2013.1899) "}],"subitem_relation_type":"references"},{"subitem_relation_type":"references","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.3892/ijo.2013.1899","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2014-11-21"}],"displaytype":"simple","filename":"k6190_3.pdf","filesize":[{"value":"683.5 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2004963/files/k6190_3.pdf"},"version_id":"5d7ccd46-7634-4699-90cf-a63f590e7d0a"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2014-11-21"}],"displaytype":"simple","filename":"k6190_1.pdf","filesize":[{"value":"184.9 KB"}],"mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://hiroshima.repo.nii.ac.jp/record/2004963/files/k6190_1.pdf"},"version_id":"2b3ee25b-0a44-48d0-b408-e01a34292fec"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2014-11-21"}],"displaytype":"simple","filename":"k6190_2.pdf","filesize":[{"value":"70.5 KB"}],"mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://hiroshima.repo.nii.ac.jp/record/2004963/files/k6190_2.pdf"},"version_id":"02e31e36-aa94-4d3f-b050-de5c1513f8a1"}]},"item_1617944105607":{"attribute_name":"Degree Grantor","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"広島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"15401","subitem_degreegrantor_identifier_scheme":"kakenhi"}]},{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Hiroshima University"}]}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"36258"},"item_title":"Involvement of ribonucleotide reductase-M1 in 5-fluorouracil-induced DNA damage in esophageal cancer cell lines","item_type_id":"40003","owner":"41","path":["1730444915582"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-11-21"},"publish_date":"2014-11-21","publish_status":"0","recid":"2004963","relation_version_is_last":true,"title":["Involvement of ribonucleotide reductase-M1 in 5-fluorouracil-induced DNA damage in esophageal cancer cell lines"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-20T07:12:39.277275+00:00"}