2026-03-12T10:51:49Z https://hiroshima.repo.nii.ac.jp/oai

oai:hiroshima.repo.nii.ac.jp:02013707 2025-06-02T03:29:16Z 1727147343679:1730442224523:1730442242406

The Sarin-like Organophosphorus Agent bis(isopropyl methyl)phosphonate Induces Apoptotic Cell Death and COX-2 Expression in SK-N-SH Cells Arima, Yosuke Yoshimoto, Kanji Namera, Akira Makita, Ryosuke Murata, Kazuhiro Nagao, Masataka Sarin Organophosphates Apoptosis COX-2 490 Organophosphorus compounds, such as sarin, are highly toxic nerve agents that inhibit acetylcholinesterase (AChE), but not cholinesterase, via multiple mechanisms. Recent studies have shown that organophosphorus compounds increase cyclooxygenase-2 (COX-2) expression and induce neurotoxicity. In this study, we examined the toxicity of the sarin-like organophosphorus agent bis(isopropyl methyl)phosphonate (BIMP) and the effects of BIMP on COX-2 expression in SK-N-SH human neuroblastoma cells. Exposure to BIMP changed cell morphology and induced caspase-dependent apoptotic cell death accompanied by cleavage of caspase 3, caspase 9, and poly (ADP-ribose) polymerase (PARP). It also increased COX-2 expression, while pretreatment with a COX inhibitor, ibuprofen, decreased BIMP-dependent cell death and COX-2 expression in SK-N-SH cells. Thus, our findings suggest that BIMP induces apoptotic cell death and upregulates COX-2 expression. http://purl.org/coar/resource_type/c_6501 Hiroshima University Medical Press 2016-03 VoR 0018-2052 AA00664312 1 Hiroshima Journal of Medical Sciences 1 65 8 1 Hiroshima Journal of Medical Sciences https://hiroshima.repo.nii.ac.jp/records/2013707 eng open access Copyright (c) 2016 Hiroshima University Medical Press