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oai:hiroshima.repo.nii.ac.jp:02007570 2025-02-21T10:12:52Z 1730444907710

Induction of non-specific suppression in chicks by specific combination of maternal antibody and related antigen Abou Elazab, Mohamed Fahmy Horiuchi, Hiroyuki Furusawa, Shuichi chick maternal antibody non-specific suppression T-cell 640 Specific immune suppression in newly hatched chicks induced by specific maternal antibodies has been reported. Laying hens were immunized with dinitrophenyl-keyhole limpet hemocyanin (DNP-KLH). Purified maternal anti-DNP and non-specific immunoglobulin (Ig) Y antibodies were transferred by yolk sac inoculation to newly hatched chicks, and then, they were immunized with an optimum immunogenic dose of DNP-KLH at 1 and 4 weeks of age. Concentrations of anti-DNP antibodies in serum samples of these chicks were measured by using Enzyme-linked immunosorbent assay (ELISA). Proportions of T-cell subsets in peripheral blood of these chicks were also measured by flow cytometric analysis at 5 weeks of age (one week after the second immunization). Suppression of anti-DNP antibody response and down-regulation of CD3+CD4+ cells were observed in the chicks received high dose of maternal anti-DNP antibodies and immunized with DNP-KLH. On the other hand, normal anti-DNP antibody response and normal proportion of CD3+CD4+ cells were observed in the chicks received high dose of non-specific IgY antibodies and immunized with DNP-KLH. Furthermore, when chicks received high dose of maternal anti-DNP antibodies and immunized with DNP-KLH at 1 and 4 weeks of age and then with rabbit serum albumin (RSA) at 5 and 8 weeks of age, their primary anti-RSA response was also significantly suppressed. We indicate here that specific maternal antibodies can affect both B and T cell responses and induce non-specific suppression against different antigens. However, this non-specific suppression does not continue for a long time. This study was supported by research project for improving food safety and animal health of the Ministry of Agriculture, Forestry and Fisheries of Japan. http://purl.org/coar/resource_type/c_6501 The Japanese Society of Veterinary Science 公益社団法人日本獣医学会 2015-06-06 VoR 1347-7439 0916-7250 AA10796138 1363 Journal of Veterinary Medical Science 11 77 1369 1363 Journal of Veterinary Medical Science https://hiroshima.repo.nii.ac.jp/records/2007570 eng 10.1292/jvms.14-0525 https://www.jstage.jst.go.jp/article/jvms/advpub/0/advpub_14-0525/_article/ open access Copyright (c) 2015 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License <http://creativecommons.org/licenses/by-nc-nd/3.0/>.