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oai:hiroshima.repo.nii.ac.jp:02006498 2025-02-21T07:42:10Z 1730444907710

Detection of living Salmonella cells using bioluminescence Urata, Masaaki Iwata, Rei Noda, Kenichi Murakami, Yuji Kuroda, Akio ATP assay Immunochromatographic lateral flow assay Mutant firefly luciferase Salmonella enteritidis 570 ATP-based bioluminescence using mutant firefly luciferase was combined with an immunochromatographic lateral flow test strip assay for Salmonella enteritidis detection. In this combination method, the Salmonella-antibody-gold complex captured at the test line on the test strip was lysed by heat-treatment, and the ATP released from the cells was measured using mutant luciferase. This method resulted in approximately 1,000 times higher sensitivity in the detection of Salmonella (i.e. 10(3) c.f.u./ml) compared to immunochromatographic lateral flow assay. http://purl.org/coar/resource_type/c_6501 Springer 2009 AO 0141-5492 AA00110183 737 Biotechnology Letters 5 31 741 737 Biotechnology Letters https://hiroshima.repo.nii.ac.jp/records/2006498 eng 10.1007/s10529-009-9924-5 http://dx.doi.org/10.1007/s10529-009-9924-5 open access Copyright (c) 2009 Springer-Verlag. "The original publication is available at www.springerlink.com"