2026-03-13T01:19:40Z https://hiroshima.repo.nii.ac.jp/oai

oai:hiroshima.repo.nii.ac.jp:02007139 2025-02-21T09:11:05Z 1730444907710

Virgibacillus salarius sp. nov., a novel halophilic bacterium isolated from a Saharan salt lake Hua, Ngoc-Phuc Hua Ngoc-Phuc Amel, Hamza-Chaffai Amel Hamza-Chaffai Vreeland, Russell H. Vreeland Russell H. Isoda, Hiroko Isoda Hiroko Naganuma, Takeshi Naganuma Takeshi open access Copyright (c) 2008 International Union of Microbiological Societies 460 A Gram-positive, endospore-forming, rod-shaped and moderately halophilic bacterium was isolated from a salt crust sample collected in Gharsa salt lake (Chott el Gharsa), Tunisia. The newly isolated bacterium designated SA-Vb1T was identified based on polyphasic taxonomy including genotypic, phenotypic and chemotaxonomic characterization. Strain SA-Vb1T was closely related to Virgibacillus marismortui and V. olivae with 16S rRNA gene sequence similarities of 99.7% and 99.4%, respectively. However, strain SA-Vb1T was distinguished from these two species on phenotypic characteristics and on the basis of DNA-DNA relatedness (29.4% and 5.1%, respectively). Genetic relationships between strain SA-Vb1T and the type species V. pantothenticus IAM 11061T and other type strains of the genus were 96-98% based on 16S rRNA gene and 18.3-22.3% based on DNA-DNA hybridization. Biochemical analysis resulted in determination of major fatty acids, iso-C15:0, anteiso-C15:0 and anteiso-C17:0 (33.3%, 29.2%, and 9.8%, respectively); phosphatidyl glycerol, diphosphatidyl glycerol, and phosphatidyl ethanolamine were the main polar lipids; and MK-7 the predominant menaquinone (~100%). The distinct characteristics demonstrated by these data show that strain SA-Vb1T represents properties of a novel species of the genus Virgibacillus. Accordingly, this isolate is proposed as Virgibacillus salarius sp. nov. The type strain is SA-Vb1T (=JCM 12946T =DSM 18441T). Society for General Microbiology 2008 eng journal article AO https://hiroshima.repo.nii.ac.jp/records/2007139 10.1099/ijs.0.65693-0 http://dx.doi.org/10.1099/ijs.0.65693-0 1466-5026 AA11409259 International Journal of Systematic and Evolutionary Microbiology International Journal of Systematic and Evolutionary Microbiology 58 2409 2409 2414 https://hiroshima.repo.nii.ac.jp/record/2007139/files/IntJSystEvolMicrobiol_58_2409.pdf 281.0 KB