2026-03-11T04:08:26Z https://hiroshima.repo.nii.ac.jp/oai

oai:hiroshima.repo.nii.ac.jp:02013296 2025-06-02T03:03:01Z 1727147343679:1730442224523:1730442319952

Automated Microanalysis of Creatinine by Coupled Enzyme Reactions Asano, Kenji Asano Kenji Iwasaki, Hiroya Iwasaki Hiroya Fujimura, Katsuyuki Fujimura Katsuyuki Ikeda, Mitsunori Ikeda Mitsunori Sugimoto, Yoshimi Sugimoto Yoshimi Matsubara, Akemi Matsubara Akemi Yano, Kazuko Yano Kazuko Irisawa, Hiroko Irisawa Hiroko Kono, Fujiko Kono Fujiko Kanbe, Masayuki Kanbe Masayuki Tsubokura, Tokuo Tsubokura Tokuo Inouye, Yoshio Inouye Yoshio Nakamura, Shoshiro Nakamura Shoshiro open access Creatinine Creatinine amidohydrolase Creatine amidinohydrolase Sarcosine oxidase 490 Hydrogen peroxide was generated from creatinine by the sequential enzyme reactions of creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase. Hydrogen peroxide was, in turn, used stoichiometrically for the condensation of 4-aminoantipyrine and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine catalyzed by horse-radish peroxidase, resulting in the formation of quinone dye with maximum absorbance at 546 nm. The optimized assay conditions for the enzymatic determination of creatinine in a HITACHI 7250 autoanalyzer was established. This system, which requires less than 5 μl of sample, was found to be the most economical for laboratories equipped with autoanalyzers. Hiroshima University Medical Press 1992-03 eng departmental bulletin paper VoR https://hiroshima.repo.nii.ac.jp/records/2013296 1572805 0018-2052 AA00664312 Hiroshima Journal of Medical Sciences Hiroshima Journal of Medical Sciences 41 1 1 1 5 https://hiroshima.repo.nii.ac.jp/record/2013296/files/HiroshimaJMedSci_41_1.pdf 414.4 KB