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oai:hiroshima.repo.nii.ac.jp:02013242 2025-06-02T03:00:55Z 1727147343679:1730442224523:1730442325010

The Effect of Phorbol Esters on Cell Growth and Epidermal Growth Factor Receptor Modulation in a Human Gastric Carcinoma Cell Line TMK-1 Ochiai, Atsushi Ochiai Atsushi Yasui, Wataru Yasui Wataru Kameda, Takashi Kameda Takashi Takanashi, Atsushi Takanashi Atsushi Takekura, Naoki Takekura Naoki Tahara, Eiichi Tahara Eiichi open access Phorbol ester Protein kinase C Gastric cancer cell EGF receptor 490 Tumor promoting phorbol esters, 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12, 13-dibutyrate (PDBu), significantly enhanced the growth of human gastric cancer cell line TMK-1, whilst activating protein kinase C. The time course of 125I-epidermal growth factor (EGF) binding to TMK-1 cells after TPA treatment showed a decrease in the number of EGF receptors on TMK-1 cells within 3 hr. Autophosphorylation of EGF receptor decreased in accordance with the decrease of EGF binding by TPA treatment. Scatchard plot analysis of TMK-1 cells after TPA treatment showed that high affinity EGF receptor disappeared at 3hr but the number of EGF receptors increased at 24 hr. These findings suggest that tumor promoting phorbol esters stimulate the cell growth through activation of protein kinase C and modification of EGF receptor of human gastric cancer cell line TMK-1. This research was supported in part by Grants-in Aid for Cancer Research from the Ministry of Education, Science and Culture of Japan and from the Ministry of Health and Welfare for a Comprehensive 10-Year Strategy for Cancer Control, Japan. Hiroshima University Medical Press 1989-12 eng departmental bulletin paper VoR https://hiroshima.repo.nii.ac.jp/records/2013242 2637248 0018-2052 AA00664312 Hiroshima Journal of Medical Sciences Hiroshima Journal of Medical Sciences 38 4 191 191 195 https://hiroshima.repo.nii.ac.jp/record/2013242/files/HiroshimaJMedSci_38_191.pdf 349.6 KB