2026-03-12T01:53:57Z https://hiroshima.repo.nii.ac.jp/oai

oai:hiroshima.repo.nii.ac.jp:02012802 2025-06-02T02:48:50Z 1727147343679:1730442224523:1730442347652

LYSOSOMAL ENZYME RELEASE FROM POLYMORPHONUCLEAR LEUKOCYTES IN PATIENTS WITH CHRONIC GRANULOMATOUS DISEASE : THE EFFECT OF HYDROGEN PEROXIDE ON RELEASED ENZYME ACTIVITIES 慢性肉芽腫症患者好中球よりのリソゾーム酵素放出について Kobayashi, Masao Kobayashi Masao Tanaka, Takeo Tanaka Takeo Usui, Tomofusa Usui Tomofusa open access 490 Lysosomal enzyme release from PMN exposed to STZ was examined using PMN from normal and CGD donors. Normal PMN showed an increase of extracellular lysosomal enzyme activity and a marked reduction of totol (extra-plus intra-cellular) enzyme activity after phagocytosis. On the other hand, in PMN from CGD patients, such a reduction of total enzyme activity was not observed and much more enzyme than normal was released extracellularly. When the supernatant from PMN from CGD patients after phagocytosis of STZ was incubated with an artificial H2O2 generating system, glucose plus glucose oxidase, the enzyme activities were greatly suppressed as the amount of H2O2 rose. A similar result was obtained with the addition of glucose oxidase during phagocytosis in PMN from CGD patients. These findings suggest that the presence of H2O2 might suppress lysosomal enzyme activities and result in inhibition of lysosomal enzyme release. This work was supported in parts by Grants-in-Aid for Scientific Research (Project Nos. 437010 and 544050) from the Ministry of Education, Science, and Culture of Japan. Hiroshima University School of Medicine 1981-12-10 eng departmental bulletin paper VoR https://hiroshima.repo.nii.ac.jp/records/2012802 6765683 0018-2052 AA00664312 Hiroshima Journal of Medical Sciences Hiroshima Journal of Medical Sciences 30 4 339 339 344 https://hiroshima.repo.nii.ac.jp/record/2012802/files/HiroshimaJMedSci_30_339.pdf 4.0 MB