2026-03-15T05:05:48Z https://hiroshima.repo.nii.ac.jp/oai

oai:hiroshima.repo.nii.ac.jp:02006840 2025-02-21T08:32:12Z 1730444907710

Thermal Structure Transformation of Methylammonium Vanadate and it's Application as a Negative Staining Reagent for Observing SARS-CoV-2 Sukmana, Ndaru Candra Sukmana Ndaru Candra Sugiarto Shinogi, Jun Shinogi Jun Yamamoto, Akima Yamamoto Akima Higashiura, Akifumi Higashiura Akifumi Sakaguchi, Takemasa Sakaguchi Takemasa Sadakane, Masahiro Sadakane Masahiro open access This is the peer reviewed version of the following article: N. C. Sukmana Sugiarto, J. Shinogi, A. Yamamoto, A. Higashiura, T. Sakaguchi, M. Sadakane, Eur. J. Inorg. Chem. 2022, e202200322., which has been published in final form at https://doi.org/10.1002/ejic.202200322. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。 Negative staining reagents Polyoxometalates Polyoxovanadates Structure elucidation Thermochemistry The solid-state thermal structure transformation of methylammonium vanadate, (CH3NH3)VO3, from −150 °C to 350 °C is reported. Variable-temperature X-ray single-crystal structure analysis at 23, 0, −50, −100, and −150 °C reveal (CH3NH3)VO3 comprises of methylammonium cations and “snake-like" ([VO3]−)n anion chains propagating along the c-direction in the Pna21 space group. In between −150 and −100 °C, we observe a reversible structural transformation due to the re-orientation of the methylammonium cations in the crystal packing, which is also confirmed by the reversible profiles observed in differential scanning calorimetry. The methylammonium vanadate is stable until at ca. 100 °C and further heating releases methylamine and water and V2O5 is formed at ca. 275 °C . Furthermore, we show that the methylammonium vanadate can be used as a negative staining reagent for visualizing SARS-CoV-2, allowing us to discern the spike proteins from the body of the virus using transmission electron microscopy. This study was supported by JST A-STEP (Grant Number JPMJTM20RF), the International Network on Polyoxometalate Science at Hiroshima University, and the JSPS Core-to-Core program. N. C. S. would like to thank the financial support from the Osimo Scholarship and Program for Developing and Supporting the Next Generation of Innovative Researchers at Hiroshima University. Wiley 2022-09-09 eng journal article AM https://hiroshima.repo.nii.ac.jp/records/2006840 https://doi.org/10.1002/ejic.202200322 European Journal of Inorganic Chemistry 2022 26 e202200322 e202200322 https://hiroshima.repo.nii.ac.jp/record/2006840/files/EJIC_26.pdf 1.9 MB